Composite

Part:BBa_K318515:Design

Designed by: Sarah R. Sandock   Group: iGEM10_Wisconsin-Madison   (2010-09-26)

acrRA + RFP + TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
    Illegal XhoI site found at 785
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1396
    Illegal AgeI site found at 1508
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The acrRA was PCR amplified from Salmonella enterica strain LT2 and was cloned in front of an RFP gene to test the construct in conjunction with the J23100+ramA construct. After many attempts with different clones, we have not been able to successfully clone it in front of the RFP construct.

Source

The Salmonella enterica strain LT2 chromosomal DNA came from Diana Downs' Lab at the University of Wisconsin-Madison.

References

Nikaido E., A. Yamaguchi, and K. Nishino. 2008. AcrAB Multidrug Efflux Pump Regulation in Salmonella enterica serovar Typhimurium by RamA in Response to Environmental Signals. J. Biol. Chem. 283:24245-24253.